At what temperature does an autoclave typically sterilize equipment?

An autoclave typically sterilizes equipment at a temperature of 250°F (121°C). This temperature is crucial because it allows the steam under pressure to penetrate the materials being sterilized, effectively killing bacteria, viruses, fungi, and spores. The combination of high temperature and pressure facilitates the denaturation of proteins and disrupts cell membranes, ensuring that microorganisms are eradicated.

This temperature is maintained for a specific duration, usually around 15-30 minutes, depending on the load size and type of materials being sterilized. Maintaining the correct temperature and time is essential for achieving proper sterilization and preventing the survival of resilient bacteria or spores.

The other temperature options listed do not provide the necessary conditions for effective sterilization. For instance, 320°F (160°C) is often used in dry heat sterilization, which operates differently than steam sterilization. Similarly, 212°F (100°C) represents boiling water, which is insufficient for sterilization as many organisms can survive that temperature. Lastly, 180°F (82°C) is also too low to ensure the destruction of all microbial life. Therefore, the accurate temperature that characterizes autoclave sterilization is indeed 250°F (121°C).